Figure 1.

CAFs inhibit drug-induced LNCaP cell death. (a) Schematic of the experimental set-up for transwell co-culture assay. (b) FACS-PI analysis showing sub-G1 cell population of LNCaP cells co-cultured with fibroblasts (CAF or NF) after 24, 48 and 72 h of 1 μM doxorubicin treatment (mean and S.E.M.; N=7; *P<0.05, **P<0.01). (c) Schematic image of the conditioned medium (CM) assay. (d) Sub-G1 cell population of LNCaP cells grown with fresh (non-CM) or fibroblast- (CAF or NF) conditioned medium (CM), as determined by FACS-PI analysis after 24, 48 and 72 h exposure of 1 μM doxorubicin (mean and S.E.M.; N=5; *P<0.05). NT, non-treated