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. 2017 Jun 1;8(6):e2844. doi: 10.1038/cddis.2017.226

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of VIP receptor in cell lines and tumors. (a) Cell lysates of indicated cells were western blotted, and the blot was developed with anti-VIPR1 antibodies. Equal loading was assessed by β-actin antibodies. (b) VIP receptor expression was measured by quantitative real-time PCR on malignant and non-malignant tumor adjacent normal tissue from breast cancer patients. In about 60% tumors VIPR1 expression was more than non-malignant tissue. (c) Protein was extracted from breast cancer tumors and 50 μg protein was western blotted, and the endogenous VIPR1 expression was detected