Figure 1.

LSD1 and HDAC inhibitors synergize to induce cell death in RMS cells. (a) Cells were treated with indicated concentrations of GSK690 and/or JNJ-26481585 for 72 h. Cell death was determined by flow cytometric analysis of DNA fragmentation of PI-stained nuclei. (b) Cells were treated with 1 μM GSK690 and 15 nM JNJ-26481585 (RD) or 10 μM GSK690 and 5 nM JNJ-26481585 (RH30) for 72 h. Cell viability was assessed by MTT assay. (c) Cells were treated with 1 μM GSK690 (RD cells) or 10 μM GSK690 (RH30 cells) and/or 15 nM JNJ-26481585 for indicated time points. Cell death was determined by flow cytometric analysis of DNA fragmentation of PI-stained nuclei. In (a-c) mean and S.D. of three independent experiments performed in triplicate are shown; *P<0.05; **P<0.01; ***P<0.001