Figure 5.

In vitro overexpression of miR-30a-5p in 769-P cells reverses EMT through regulation of ZEB2. (a) Expression of epithelial and mesenchymal markers was measured by western blot in wild-type, miR-control and miR-30a-5p 769-P cells. GAPDH was used as a loading control. (b) ZEB2 was transfected into miR-30a-5p 769-P cells. Western blot demonstrating that miR-30a-5p inhibition of EMT was abrogated in miR-30a-5p 769-P cells upon ectopic expression of ZEB2. (c) Wound healing and (d) invasion assays showing ectopic expression of ZEB2 in miR-30a-5p 769-P cells abrogated miR-30a-5p suppression of migration and invasion of 769-P cells