Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 15;8(6):e2886. doi: 10.1038/cddis.2017.255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Disruption of autophagy impairs DHA-induced GATA6 accumulation and HSC senescence in vitro. Activated HSCs were stably transfected with Atg5 siRNA or Atg5 plasmid, and then were treated with the indicated concentration of DHA for 24 h. (a, b) The transfection efficiency was confirmed by western blot analysis. (c) β-galactosidase staining analysis was used to detect HSC senescence. (d) Real-time PCR analyses were used to determine the expression of p53 and p16. (e) GATA6 and p53 immunostaining were used to determine the expression of GATA6 and p53. For the statistics of each panel in this figure, data are expressed as mean±S.D. (n=3); *P<0.05 versus control, **P<0.01 versus control, ***P<0.001 versus control. ^#P<0.05 versus DHA treatment, ^##P<0.01 versus DHA treatment, ^###P<0.001 versus DHA treatment