Figure 2.

CRNDE depletion inhibits cell proliferation, cell cycling, invasion and migration in CRC cells in vitro. (a) DLD1 and HCT116 cells were infected with Lv-siCRNDE to establish two cell lines with stable knockdown of CRNDE expression. CRNDE levels in DLD1 and HCT116 cells after shRNA-mediated knockdown of CRNDE were detected by real-time RT-PCR. (b) CCK-8 assays were performed to determine the proliferation of CRNDE-depleted CRC cells. (c) Colony-forming assays were performed to determine the effects of CRNDE depletion on the growth of CRC cells. (d) Cell cycle progression was analyzed by flow cytometry. (e) Apoptosis assays were performed to determine the effects of CRNDE depletion on CRC cells by flow cytometry (upper) and by western blot analysis (lower). (f) Matrigel invasion assays were used to determine the effects of CRNDE depletion on the invasion ability of CRC cells. (g) Scratch-wound-healing assays were performed to determine the effects of CRNDE depletion on the migration ability of CRC cells. The experiments were performed in triplicate, and the data are expressed as the mean±S.D. *P<0.05, **P<0.01 and ***P<0.001