Figure 3.

The CRNDE depletion inhibits the growth and metastasis of human CRC cells in mice in vivo. (a) DLD1 cells with stable CRNDE downregulation and control cells were inoculated into nude mice. These graphs show the tumor xenografts 4 weeks after ectopic-subcutaneous implantation in nude mice. (b) DLD1 cells with downregulated CRNDE expression exhibited attenuated tumor growth in nude mice. The effect of CRNDE on CRC tumor growth was evaluated based on tumor volume in the two groups. (c) Representative pictures of lung metastasis by hematoxylin and eosin (H&E) staining in nude mice 4 weeks after tail vein injection with CRNDE downregulated DLD1 cells or control cells. (d) Statistical comparisons of lung metastasis and pulmonary tumor colonies per view in the two groups of mice after tail vein injection