Figure 1.

OA decreased the intracellular lipid level of colon cancer cells under hypoxia. HCT116 cells were cultured in the McCoy’s 5A culture medium containing 10% FBS and 50 μM sodium palmitate (PA) under hypoxia. Cells were treated with OA (25, 50, 100 and 150 μM) for 36 h and then collected. (a) Cells were stained with oil red and observed. (b) Cells were stained with nile red and observed under confocal microscopy (× 400). (c) Cells were stained with nile red. Then fluorescence intensity was quantified by flow cytometry at Ex/Em of 552/636 nm. (d) The intracellular free fatty acid was assayed with the Free Fatty Acid Quantification Kit. Bars, S.D.; ^#P<0.05 versus untreated controls in hypoxia; *P<0.05 or **P<0.01 versus PA treated alone in hypoxia