Figure 3.

OA induces cell cycle arrest and suppresses the growth of colon cancer cells under hypoxia. HCT116 cells were cultured in the McCoy’s 5A culture medium containing 10% FBS and 50 μM PA, and treated with OA for 36 h under hypoxia. (a) Cell growth inhibitory rates were measured by MTT assay. (b) Cells were fixed, permeabilized and stained with Ki67 assay kit. (c) Cell cycle distribution was monitored by flow cytometry. (d) The cell cycle distributions were quantified. (e) The colony formation ability was investigated after culturing at 37 °C, 5% CO₂ for 28 days. The pictures of colonies in a six-well dish were taken with phase contrast microscopy. (f) Western blot assays were performed for cycle-related protein, including p21, cyclin B, CDK1 and p-CDK1 (Thr161)