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. 2017 Jun 15;8(6):e2875. doi: 10.1038/cddis.2017.264

Figure 2.

Figure 2

(a) Immunofluorescence (IF) analysis of RAD51. RAD1 foci induction by IF after MMC treatment in MMC sensitive SB1690CB, MMC-resistant SBRes and BRCA2 competent K562 control cells. Absence of foci in SB1690CB, and presence of foci in SBRes and control K562 cells as indicated. γH2AX foci formation as control for DNA damage. (b) Numerical evaluation of RAD51 foci induction after MMC comparing sensitive SB1690CB cells and resistant SBRes cells (statistical analysis: One way-ANOVA and Tukey post-test *P<0.05, **P<0.01). Increase of γH2AX foci formation after MMC as control for DNA damage. (c) Western blot analysis of BRCA2. Antibody recognizing the BRCA2 epitope as indicated (upper panel) for western blot analysis of BRCA2 in MMC sensitive SB1690CB cells, SRes cells with acquired MMC résistance. HEK293 as BRCA2-WT and CAPAN1 cells as negative control for full-length BRCA2. (d) Quantification of BRCA2 signal from four biological repeats (statistical analysis: T-test, **P<0.01)