Figure 5.

EGR-1/ASPP1 promotes Quercetin-induced apoptosis. (a) SiRNA-mediated ASPP1 or EGR-1 knockdown was confirmed by WB. iASPP was a negative control. (b) MTT assays were applied to compare the viability of the cells after ASPP1 or EGR-1 knocking down. The values were derived from three independent experiments; S.D. were shown as error bars. (c) The cells were treated with Quercetin, stained with Annexin V/PI and subjected to the FACS analysis. The average of apoptotic cells (Annexin V positive) was derived from three independent experiments; S.D. are shown as error bars. *P<0.05, in comparison with untreated control; ^#P<0.05, in comparison with Quercetin-treated Si-control cells. (d) Cells were treated with 80 μM Quercetin and 50 μM or 100 μM autophagy inhibitor, chloroquine (CQ) for 24 h. Then they were collected and stained with Annexin V/PI and subjected to the FACS analysis. The average of apoptotic cells (Annexin V positive) was derived from three independent experiments; S.D.are shown as error bars. *P<0.05, in comparison with CQ-untreated control