Figure 7.

EGR-1/ASPP1 inhibits cyto-protective autophagy by compete Atg5–12 with Atg6 to form a complex. (a) The expression of ASPP1, EGR-1 and Atg5–12 were determined by WB analysis in the presence or absence of Quercetin in HCT116/Shnon and HCT116/ShASPP1 stable lines. β-Actin was used as a loading control. (b) The expression of ASPP1, EGR-1 and Atg5–12 were determined by WB analysis in the presence or absence of Quercetin in HCT116 cells transfected with Si-Control or Si-EGR-1. β-Actin was used as a loading control. (c) ASPP1 was re-expressed after SiRNA-mediated EGR-1 knockdown. The expression levels of ASPP1, EGR-1 and Atg5–12, were determined by WB. β-Actin was used as a loading control. (d) Cell lysates derived from HCT116/Shnon and HCT116/ShASPP1 cells were immunoprecipitated with anti-Atg16 antibody. The co-precipitated ASPP1 or Atg5–Atg12 was evaluated by WB. *P<0.05