Figure 3.

FAM210B expression modulates cancer cell invasion and migration. (a) Western blotting for FAM210B in the indicated cells/treatment. (b) Colony formation by the indicated cells. (c) Crystal violet-stained SKOV3 and A549 cells following invasion and (d) quantitation of invasion assay. (e) Light microscopic imaging (scale bar: 200 μm) of migrated cells in the scratch assay. (f) SKOV3-luc-GFP and SKOV3-Luc-GFP-shFAM210B cells were injected under the bursal membrane of the ovary, and tumor weight was measured at the experimental end point (SKOV3-luc-GFP, n=6 mice; SKOV3-Luc-GFP-shFAM210B, n=6 mice, unpaired two-tailed Student’s t-test). (g) Representative images of mice harboring orthotopic SKOV3-luc-GFP and SKOV3-Luc-GFP-shFAM210B tumors. The primary tumors were indicated with white lines and the metastatic tumors were indicated with white arrows. (h) Number of peritoneal cavity metastasis nodules in mice with orthotopic SKOV3-luc-GFP and SKOV3-Luc-GFP-shFAM210B tumors. Data were represented as the means±S.E.M. NS: not significant. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001