Figure 3.

Evidence for the involvement of NADPH oxidase-derived ROS in Aβ_1–42 toxicity. (a) Effect on cell viability of a 24 h exposure of astrocytes to Aβ_1–42 alone (10–500 nM, white bars) or Aβ_1–42 in the additional presence of 100 μM MnTMPyP (grey bars). Bars represent the mean±S.E.M. data of cells from 3 repeats (each performed in duplicate) with cells from different passages. (b) as (a), except cells were either treated with Aβ_1–42 alone (10–500 nM, white bars) or Aβ_1–42 in the additional presence of 10 μM Mito-TEMPO (grey bars). Bars represent the mean±S.E.M. data of cells from 3 repeats (each performed in duplicate). Significance: ***P<0.001 as compared with respective controls, or between cells without drug versus MnTMPyP (a). NS, not significant. (c) Effect on cell viability of a 24 h exposure of astrocytes to Aβ_1–42 alone (100–1000 nM, white bars) or Aβ_1–42 in the additional presence of 1 mM apocyanin (grey bars). Bars represent the mean±S.E.M. data of cells from 3 repeats (each performed in duplicate) with cells from different preparations. (d) as (c), except cells were either treated with Aβ_1–42 alone (100–1000 nM, white bars) or Aβ_1–42 in the additional presence of 10 μM GKT137831 (grey bars). Bars represent the mean±S.E.M. data of cells from 2 repeats (each performed in duplicate) with cells from different preparations. Significance: **P<0.01; ***P<0.001 as compared either with respective controls, or between drug treatment or no treatment, as indicated