Figure 8.

Hypoxia protects against Aβ_1–42 toxicity via HO-1 induction. (a) Representative images of astrocytes immunostained for HO-1 under control conditions, or following a 48 h exposure to hypoxia (0.5% O₂) as indicated. Scale bar applies to both images. (b) Example of western blot showing induction of HO-1 by hypoxia (hyp., 48 h, 0.5% O₂). Bar graph plots mean±S.E.M. (n=3) densitometry (relative to control (normoxia)) taken from blots. *P<0.05. (c) Effect on cell viability of a 24 h exposure of astrocytes to Aβ_1–42 alone (100–1000 nM, white bars) or Aβ_1–42 under hypoxic conditions in the absence (grey bars) or additional presence of 10 μM QC-15 (black bars). Bars represent the mean±S.E.M. data of cells from 3 repeats (each performed in duplicate) with cells from different preparations. Significance: **P<0.01; ***P<0.001 as compared with control or as compared between treatments, as indicated