Figure 1.

shRNA screen for TRAIL regulating CMEs in GBMs. (a) Schematic diagram of the experimental setup for the shRNA TRAIL screen in U87MG cells. Cells were individually infected with 125 different shRNAs targeting CMEs and selected with puromycin. Viability analysis was conducted after 100 ng/ml TRAIL treatment for 24 h. (b) Table of categorized 48 different CMEs targeted in the shRNA screen. (c) Percent viability analysis of the untreated cells after infection. (d) Percent change viability analyses of the infected and TRAIL-treated cells. (e) Top sensitizer hits below 2 S.D. Data were normalized to shControl cells. Red bar indicates shControl. Genes lying below and over that of shControl were classified as suppressors and enhancers of apoptosis, respectively. Dotted lines indicate 2 S.D. from % viability change of shControl cells