Figure 4.

KDM2B regulates apoptotic machinery at transcriptional level in GBM cells. (a) Ingenuity pathway analysis of RNA-seq results. (b) List of the top 10 canonical pathways that were most significantly upregulated or downregulated upon KDM2B loss. (c) Custom list of genes displaying top deregulated apoptosis-related genes along with transcript level of KDM2B (n=2 biological replicates, average number of reads of shKDM2B cells were normalized to shControl cells). (d) qRT-PCR validation of HRK gene expression levels of shKDM2B cells. Expression levels were normalized to shControl. (e) Western blots showing HRK upregulation in shKDM2B cells at the protein level (f) qRT-PCR quantification of HRK mRNA level of HRK knockdown cells. Expression levels were normalized to shControl. (g) Cell viability analysis upon TRAIL treatment of shControl and shHRK cells transduced with either shControl or shKDM2B. Data were normalized to untreated cells of each group. (*,** and *** denotes P<0.05, P<0.01 and P<0.001, two-tailed Student’s t-test). All experiments were conducted with U87MG cells with 100 ng/ml TRAIL treatment