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. 2017 Jun 29;8(6):e2911. doi: 10.1038/cddis.2017.299

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Autophagosomes are formed in cortical cultures during GD and late GR. Representative images of time-lapse autophagosome formation in cortical cultures exposed to GD/GR using the Cyto-ID detection kit (green) and Hoechst counterstaining (blue) (a and b). Asterisks indicate magnification zones. Graph shows the number of autophagosomes at different times after GD/GR (c). Bars represent mean±S.E.M. (n=3–6). Data were analyzed by one-way ANOVA followed by a Fisher’s post hoc test *P<0.05 versus control