Figure 1.

Effect of ochratoxin A (OTA) on the cell viability and ATP contents of PK-15 cells. PK-15 cells were seeded in 96-well plates at a density of 5 × 10³ cells/well or in 24-well plates at a density of 4 × 10⁴ cells/well and cultured in the presence of 0.01, 0.1, 1, 2, 5, or 10 μM OTA for 48 h. (a) Cell viability was determined by MTT assay. (b) Intracelluar ATP concentrations were determinded using a luciferase-based ATP assay kit. The data are presented as means±S.E. of three independent experiments. Statistical significance compared with the control is indicated by *P<0.05 and **P<0.01