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. 2017 Jun 29;8(6):e2909. doi: 10.1038/cddis.2017.303

Figure 5.

Figure 5

Inhibition of autophagy with 3-MA reverses PCV2 replication induced by OTA in PK-15 cells. PCV2-infected cells were incubated with OTA (0.1 μM) with or without 3-MA (5 mM). The cells were then assayed for (a,b) expression levels of LC3, Cap and β-actin (loading control), (c) PCV2 viral titers, (d) PCV2 viral DNA copies and (e) the number of infected cells, as described in Materials and methods. The data are presented as means±S.E. of three independent experiments. Statistical significance compared with the control is indicated by *P<0.05 and **P<0.01. Statistical significance compared with OTA is indicated by #P<0.05 and ##P<0.01