Figure 5.

Exogenous IFNγ promotes CD8 T-cell motility and enhances cytotoxicity. (a) Co-cultures of EGFP⁺OT-I CTL and SIINFEKL-loaded or untreated KC were imaged for 30 h by fluorescence time-lapse microscopy. Recombinant IFNγ was added at time 0. Representative images of tracks created by CTL as they traveled among KC targets have been mapped by software. Vector displacement of the tracks is shown by white arrows. Length of the traveled tracks and mean speed of travel is indicated by the colored legend bars (scale bars, 50 μm). (b) Translated travel tracks from (a) have been plotted from origin showing the distance and spread of the path traveled by the cells within the field of view. Each plot shows the result for one typical experiment of three independent experiments. (c–e) Displacement (c), distance traveled (d) and speed (e) of CD8 T cells in co-culture with antigen-loaded target KC. Average values of four movies are shown (>400 cells). Error bars are S.E.M. (*P<0.05, n.s. not significant)