Table 3. NMR data of actinomycin D (CDCl3, 1H: 500 MHz, 13C: 75.4 MHz).
| α-ring | Position | δC | δH | β-ring | Position | δC | δH |
|---|---|---|---|---|---|---|---|
| Thr | 1 | 168.51 | Thr | 1 | 167.55 | ||
| 2 | 55.26 | 4.62 (dd) | 2 | 54.90 | 4.52 (dd) | ||
| 3 | 75.05 | 5.21 (qd) | 3 | 74.98 | 5.18 (qd) | ||
| 4 | 17.76 | 1.26 (3H, d) | 4 | 17.34 | 1.25 (3H, d) | ||
| NH | 7.16 (d) | NH | 7.76 (d) | ||||
| D-Val | 1 | 173.74 | D-Val | 1 | 173.32 | ||
| 2 | 58.90 | 3.56 (dd) | 2 | 5873 | 3.53 (dd) | ||
| 3 | 31.82 | 2.11 (m) | 3 | 31.55 | 2.07 (m) | ||
| 4 | 19.26 | 1.13 (3H, d) | 4 | 19.08 | 1.12 (3H, d) | ||
| 5 | 19.03 | 0.91 (3H, d) | 5 | 18.98 | 0.89 (3H, d) | ||
| NH | 8.15 (d) | 8.01 (d) | |||||
| Pro | 1 | 173.37 | Pro | 1 | 173.32 | ||
| 2 | 56.44 | 6.03 (d) | 2 | 56.26 | 5.96 (d) | ||
| 3 | 31.29 | 1.89, 2.65 (m) | 3 | 30.97 | 1.85, 2.65 (m) | ||
| 4 | 23.00 | 2.15, 2.23 (m) | 4 | 22.85 | 2.15, 2.23 (m) | ||
| 5 | 47.59 | 3.74 (2H, dd) | 5 | 47.33 | 3.84 (2H, m) | ||
| Sar | 1 | 166.53 | Sar | 1 | 166.32 | ||
| 2 | 51.39 | 4.82, 3.64 (d) | 2 | 51.39 | 4.71, 3.60 (d) | ||
| NMe | 34.91 | 2.91 (3H, s) | NMe | 34.85 | 2.88 (3H, s) | ||
| MeVal | 1 | 167.65 | MeVal | 1 | 166.53 | ||
| 2 | 71.45 | 2.68 (m) | 2 | 71.28 | 2.68 (m) | ||
| 3 | 26.92 | 2.68 (m) | 3 | 26.92 | 2.68 (m) | ||
| 4 | 21.66 | 0.96 (3H, d) | 4 | 2,156 | 0.95 (3H, d) | ||
| 5 | 19.21 | 0.75 (3H, d) | 5 | 19.08 | 0.74 (3H, d) | ||
| NMe | 39.27 | 2.94 (3H, s) | NMe | 39.14 | 2.88 (3H, s) | ||
| Chromophore | |||||||
| δH 2.56 (3H, s), 2.68 (3H, s), 7.37 (d), 7.64 (d) | |||||||
| δC 7.74, 15.00, 101.75, 113.52, 125.80, 127.59, 129.13, 130.25, 132.65, 140.50, 145.12, 145.90, 147.64, 166.53, 168.99, 179.12 | |||||||