Table 1.

Percentage of positive cells expressed as a receptor expression ratio by M1-MDM and M2a-MDM vs. control MDM.

Marker	No.°	Expression by control MDM (Range)	M1 vs. control ratio (Mean ± SD)	M2a vs. control ratio (Mean ± SD)	M1 vs. control	M2a vs. control	M1 vs. M2a
CD4	6	33–59	0.14 ± 0.08	0.32 ± 0.08	**	**	*
CD14	4	73–97	1.00 ± 0.24	0.89 ± 0.29
CD16	4	36–59	0.09 ± 0.11	0.92 ± 0.13	**		*
CD18	4	91–95	0.87 ± 0.39	1.14 ± 0.05
CD80	4	25–36	1.06 ± 0.06	1.13 ± 0.12
CD86	4	60–76	0.99 ±0.03	1.10 ± 0.12
CD163	4	59–72	0.57 ±0.10	0.98 ± 0.02	*		*
CD206	4	50–57	0.57 ± 0.10	0.98 ± 0.02	*		*
HLA-DR	4	83–97	1.07 ± 0.06	1.00 ± 0.72
DC-SIGN	6	5–17	0.34 ± 0.11	9.08 ± 2.93	*	**	**

The percentage of positive cells was determined by flow cytometry after 18 h of culture in polarizing (M1 and M2a) or nonpolarizing conditions (control). ANOVA, analysis of variance; DC-SIGN, dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin; MDM, monocyte-derived macrophage.

^*P ≤ 0.05,

^**P ≤ 0.001 as assessed by one-way ANOVA and Tukey post-test. No. identifies the number of independent monocyte donors.