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. Author manuscript; available in PMC: 2018 Jan 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Dec 16;56(4):1059–1063. doi: 10.1002/anie.201610209

Fig. 1. ZNPs enable permanent CRISPR/Cas-mediated DNA editing.

Fig. 1

(A) Sequence specific silencing of luciferase by siRNA (9 nM) and editing by sgRNA (7 nM) in HeLa-Luc-Cas9 cells. N = 4 ± stdev, **** p < 0.0001 (B) Kinetically, silencing with siRNA is transient while sgRNA delivery results in permanent loss of luciferase signal after 2 days. (C) Sequence specific editing of luciferase was confirmed by the Surveyor assay. (D) The chemical structure of ZA3-Ep10.