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. Author manuscript; available in PMC: 2018 Jan 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Dec 16;56(4):1059–1063. doi: 10.1002/anie.201610209

Fig. 5. ZNPs enabled non-viral CRISPR/Cas editing in vivo.

Fig. 5

(A) Schematic representation shows that co-delivery of Cas9 mRNA and sgLoxP deletes the stop cassette and activates downstream tdTomato protein. (B) After administration of ZNPs encapsulating Cas9 mRNA:sgRNA (4:1, wt) at 5 mg/kg total RNA, tdTomato fluorescence was detected in the liver and kidney upon whole organ ex vivo imaging. (C) Confocal fluorescence microscopy of tissue sections showed tdTomato positive cells in liver, lung, and kidneys. Scale bars = 50 μm).