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. 2017 Jul 19;10(5):734–743. doi: 10.1016/j.tranon.2017.06.009

Figure 1.

Figure 1

Neutralizing antibodies inhibit THP1 cell migration induced by recombinant human CCL2 protein. (A) THP1 cells were stimulated with increasing concentrations of human (hCCL2) or mouse (mCCL2) recombinant CCL2 protein and analyzed for Transwell migration after 2 hours. Representative images of cells migrated into lower chamber are shown. (B) THP1 cells were stimulated with 10 ng/ml hCCL2 or mCCL2 in the presence or absence of increasing doses of CCL2-neutralizing antibodies or control IgG and analyzed for Transwell migration. Representative images of cells treated with CCL2 and 1 μg/ml control IgG or CCL2-neutralizing antibodies. Statistical analysis was performed using one-way ANOVA test with Bonferonni post hoc comparison. Statistical significance was determined by *P < .05, ***P < .01, and ns = not significant. Mean ± SEM is shown. Scale bar = 400 μm.