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. 2017 Jul 21;8:1374. doi: 10.3389/fmicb.2017.01374

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Copyright © 2017 Trdá, Barešová, Šašek, Nováková, Zahajská, Dobrev, Motyka and Burketová.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LmAK is involved in fungal virulence and growth. (A,B) Infection of JN3 and s.LmAK mutants on cotyledons of B. napus cv. Eurol 9 days post inoculation (dpi). (A) The panel shows representative infected cotyledons. (B) Fungal virulence evaluated as relative lesion area on cotyledons relative to JN3 isolate. Asterisks indicate significant differences between transformants and JN3 (^∗P < 0.05, ^∗∗P < 0.01). (C) Macroscopic (up) and microscopic (bottom) phenotype of the 9-day old fungal colony grown on V8 agar plates. Scale bars = 10 μm. (D) Growth on V8 plates assessed at 9 days as fresh mycelial weight (FW). All data are means ± SE from at least three independent experiments.