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. 2017 Jul 21;8:1374. doi: 10.3389/fmicb.2017.01374

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Copyright © 2017 Trdá, Barešová, Šašek, Nováková, Zahajská, Dobrev, Motyka and Burketová.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of LmAK silenced mutants of L. maculans. (A,B) Wild-type L. maculans (JN3) and different silenced LmAK (s.LmAK) transformants were cultivated in a liquid culture in vitro and mycelium was sampled at 9 days. (A) Expression of LmAK gene was analyzed by qPCR, normalized to LmTUB and reported to WT. (B) cZ content in mycelium expressed as pmol g^-1 DW ± SE. (C,D) Content of CK ribosides and monophosphates in wild-type (gray bars) and s.LmAK-105 transformant (black bars) in (C) non-treated cultures or (D) after treatment with iP (1 μM). Means ± SE from three independent experiments are shown for each assay. Asterisks indicate significant differences between transformants and JN3 (^∗P < 0.05, ^∗∗P < 0.01).