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. 2017 Jul 21;18:347. doi: 10.1186/s12859-017-1759-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Effect of bin size on signal and noise of FACS-based screens. For FACS-based screens, the effect of the size of the sorted bins (see Fig. 1) on metrics for signal, noise, and signal-to-noise ratio (scaled within each plot) is shown. Metrics are defined in the Implementation section. Simulations were run for 100× representation (top row) or 1000× representation (bottom row) at the transfection, bottleneck and sequencing stages. Lines and light margins represent means and 99% confidence intervals, respectively, for 25 independent simulation runs