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. Author manuscript; available in PMC: 2018 Jun 17.
Published in final edited form as: Biochem Biophys Res Commun. 2017 May 5;488(1):153–158. doi: 10.1016/j.bbrc.2017.04.158

Figure 3. N-linked glycosylation is abnormal in some truncated Dfi1 proteins.

Figure 3

Crude lysates of C. albicans encoding HA-tagged full-length Dfi1, Dfi1ΔC, Dfi1ΔN, or Dfi1ΔNΔC were either mock-treated (−) or treated (+) with PNGase F, and these samples were probed for HA by Western blotting, as described in the Materials and Methods. Arrow indicates a faster-migrating species. C. albicans strains encoding DFI1N-TM replacement C or DFI1N-TM replacement Z underwent identical treatment. The lanes from the DF11ΔC strain were each loaded with 11uL of sample, and all other lanes were each loaded with 45 μL of sample. Each blot is representative of at least three experiments.