Table 3. Discordant cases according to ProMisE subclassfication.

Case	Specimen	Pathology	MMR IHC	POLE	p53	Initial Assignment	Reassessment	Final ProMisE	Retest
1	Diagnostic	CAH + gr 1 EM	intact	no mut	abn	p53 abn	Retest: No POLE mut found	p53 abn	Discordant
	Hysterectomy	gr 1 EM, stage IB	intact	P286S*	abn	POLE EDM	Retest validates	POLE EDM
2	Diagnostic	gr 2 EM	MSH6 loss	no mut	wt	MMR-D	Retest: Δ to intact	p53 wt	Concordant
	Hysterectomy	gr 2 EM, stage IB	intact	no mut	wt	p53 wt	Retest: confirms intact	p53 wt
3	Diagnostic	gr 1 EM	MSH2/MSH6 loss	no mut	wt	MMR-D	Retest: changed to intact	p53 wt	Concordant
	Hysterectomy	gr 2 EM, stage IA	intact	no mut	wt	p53 wt	Retest confirms intact	p53 wt
4	Diagnostic	gr 3 undiff	MSH2/MSH6 loss	P441L	abn	MMR-D	Retest confirms MSH2/MSH6 loss	MMR-D	Concordant
	Hysterectomy	gr 3 SC, stage IB	intact	P441L	abn	POLE EDM	Retest:Δ to MSH2/MSH6 loss	MMR-D
5	Diagnostic	gr 1 EM	intact	no mut	wt	p53 wt	Retest confirms intact	p53 wt	Discordant
	Hysterectomy	gr 3 EM, stage IB	MLH1/PMS2 loss	no mut	wt	MMR-D	Retest confirms loss of MLH1	MMR-D
6	Diagnostic	gr 1 EM	intact	P286R	wt	POLE EDM	Retest validates	POLE EDM	Discordant
	Hysterectomy	gr 1 EM, stage IA	intact	no mut	wt	p53 wt	Retest: No POLE mut found	p53 wt