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Oxidative stress in the bone was measured by immunohistochemistry for protein-nitrotyrosine adducts (brown staining). Representative images (200x original magnification) showing the area directly under the tibial growth plate from bones of PF-control mice, EtOH-fed mice, NAC-fed, NAC+EtOH fed, Vit.E-fed, and Vit.E+EtOH-fed mice.
