Table 1.
Representative CE enzyme assay screens and demonstrations
| Target | Assay Type | Compounds Assayed | Z-score | Reference |
|---|---|---|---|---|
| tyrosine phosphatase | commercial microchip platform | 12,648 | 0.61 | [8] |
| Sirt5 | segmented flow coupled to MCE | 1,280 | 0.8 | [16] |
| glycerol kinase | EMMA | 1 | N.D. | [88] |
| aminopeptidase N | EMMA | 30* | N.D. | [86] |
| neuraminidase | EMMA | 24* | N.D. | [83] |
| β-N-acetylhexosaminidase | TDLFP | 1 | N.D. | [92] |
| four human kinases: GSK3β, DYRK1A, CDK5/p25, CDK1/cylcin B | TDLFP | 13 | N.D. | [93] |
| adenosine deaminase and xanthine oxidase | CE-based IMERs | 20* | 0.82, 0.74 | [101] |
| L-glutamic dehydrogenase | CE-based IMERs | 26* | 0.95 | [100] |
| glucose-6-phosphate dehydrogenase | CE-based IMERs | 6 | N.D. | [97] |
| alkaline phosphatases | CE-based IMERs | 3 | N.D. | [96] |
| acetylcholinesterase | CE-based IMERs | 46* | 0.9 | [95] |
| angiotensin-converting enzyme | CE-based IMERs | 34* | N.D. | [94] |
Not determined (N.D.); electrophoretically mediated microanalysis (EMMA); transverse diffusion of laminar flow profiles (TDLFP); capillary electrophoresis-based immobilized enzyme reactors (CE-based IMERs)
*
includes crude product, natural extract, or chinese traditional herb