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. 2017 Jun 11;26(8):1639–1652. doi: 10.1002/pro.3202

Figure 1.

Figure 1

N‐terminal methylation patterns of wild type and mutant NRMT1. The directed mutation of aromatic residues Y19 and W20 in NRMT1 and the Q144H mutation found in human lung cancer showed no effects on (A) monomethylase/dimethylase (me1/2RCC1) or (B) trimethylase (me3RCC1) activity. However, the NRMT1 mutants N209I (endometrial cancer) and P211S (lung cancer) exhibit (A) increased monomethylation/dimethylation of RCC1 and (B) decreased trimethylation of RCC1 as compared to wild type (WT). Total RCC1 is shown as loading control. (C) Densitometry analysis of panel A. Ratio of me1/2RCC1:Total RCC1 band intensity. (D) Densitometry analysis of panel B. Ratio of me3RCC1:Total RCC1 band intensity, normalized to WT NRMT1. Each data point represents the mean ± SEM of three independent experiments. * denotes P < 0.05, determined by an unpaired two‐tailed Student's t‐test. Bands were quantified using ImageJ software (NIH).