Table 1.
Strain | Transgenic F1 | Transgenic F2 | % RNAi Knockdown in F4 Transformantsa |
---|---|---|---|
AF16 | 18/22 | 10/22 | 88 ± 4 |
DH1300 | 7/25 | 4/25 | 84 ± 3 |
The extent of RNAi-mediated knockdown of daf-21 transcripts was calculated relative to the expression levels of worms fed E. coli carrying the empty vector pL4440. The values represent the means and standard errors of 3 replicates of 10 pooled worms. The number of worms depicted are those expressing DsRed in the F1 and F2 worms, relative to the number of surviving injectants.