Table 2. Oligonucleotide primers used in this study.

Primers	Sequence (5'-3')
Gene deletions
KP1_4563-A	ATAAGAATGCGGCCGCGGCGATGCTGATTTATGC
KP1_4563-B	CCCTCTGCAACCATTCGCGTTTGCTTTCGATGGACTT
KP1_4563-C	AAGTCCATCGAAAGCAAACGCGAATGGTTGCAGAGGG
KP1_4563-D	ATAAGAATGCGGCCGCTCGGGGCGATCAGTATGG
Complementation of mutant
KP1_4563-HB-KpnI-F	CGGGGTACCAGGAGGAATTCACCATGCTTTCCACCATAAAA
KP1_4563-HB-SalI-R	ACGCGTCGACTTATTTAGACAGCTCGGC
qRT-PCR
KP1_4563-RT-F	CGGTATGCTCTCCCTGGTC
KP1_4563-RT-R	TATTTAGACAGCTCGGCGGTC
LacZ fusion
KP1_4563-LacZ-F	CGCGGATCCCGATGCTGATTTATGCCAC
KP1_4563-LacZ-R	GGAAGATCTATACCGGCAGCTGCGAGTAA
Protein production
KP1_5071-CRP-P-F	GCGGGATCCATGGTGCTTGGCAAACCG
KP1_5071-CRP-P-R	GCGAAGCTTTTAACGGGTGCCGTAGACG
EMSA
KP1_4563-EMSA-F	CGATGCTGATTTATGCCAC
KP1_4563-EMSA-R	ATACCGGCAGCTGCGAGTAA
KP1_16S -EMSA-F	CGGTCTGTCAAGTCGGATGTG
KP1_16S -EMSA-R	CGGAAGCCACGCCTCAAG
DNase I footprinting
KP1_4563-FP-F	ATGTGATACCCCCTTTCAGAAG
KP1_4563-FP-R	ATACCGGCAGCTGCGAGTAA

Amplification of the KP1_4563 coding region together with AGGAGG, which is a ribosome binding site (underlined) consensus sequence, and AATTCACC (italic), a spacer. Bold letters indicate the respective restriction enzyme site in the primer.