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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: Gene. 2017 May 25;626:309–318. doi: 10.1016/j.gene.2017.05.048

Fig. 1. Alcohol induces RNA Pol III-dependent transcription.

Fig. 1

AML-12 cells and MCF-10A cells were starved in FBS/DMEM-F12 for 3h. Cells were treated with or without different amounts of ethanol as indicated. RNAs were isolated from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (A and B, left panel), 5S rRNA (A and B, right panel). The fold change was calculated by normalizing to the amount of GAPDH mRNA. High peak of the induction of Pol III genes are in 50mM ethanol of Liver cells and at 25mM ethanol of breast cells. *: P > 0.05. The columns represent Mean ± SE of at least three independent determinations.