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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: Gene. 2017 May 25;626:309–318. doi: 10.1016/j.gene.2017.05.048

Fig. 2. Pol III gene transcription is increased by alcohol.

Fig. 2

(A–D): Ethanol increases Pol III gene transcription in liver cells. Non-tumor mouse liver line, AML-12 cells and PMH (primary mouse hepatocytes) (A–B), and liver tumor cells (C–D), HepG2 and TSCML (tumor stem cells of mouse liver) were grown to 85% confluency and starved in DMEM-F12 for 3 h and treated with 50mM ethanol for another hour. (E–H): Ethanol induces Pol III gene transcription in breast cells. Non-tumor breast cell lines (MCF-10A and MCF-12A) (E–F) and ER+ breast cancer cell lines (MCF-7 and T47D) (G–H) were treated with 25mM ethanol as described above. The total RNAs from these cells were extracted to measure pre-tRNALeu, 5S rRNA and GAPDH transcripts. The fold change was calculated by normalizing to the amount of GAPDH. *: P > 0.05. The columns represent Mean ± SE of at least three independent determinations.