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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: Gene. 2017 May 25;626:309–318. doi: 10.1016/j.gene.2017.05.048

Fig. 4. Alcohol induces activation of JNK1.

Fig. 4

(A and B): Ethanol induces JNK1 activation. HepG2-ADH cells and MCF-7 cells were treated with or without ethanol as described above. Immunoblot analysis was performed using protein lysates derived from these cells and antibodies against phosphorylated JNK1/2 (46kD/54kD), JNK1/2 and β-actin as designated. (C and D): JNK1 siRNA decreases cellular levels of JNK1. HepG2-ADH cells and MCF-7 cells were transfected with mismatch RNA (siMM) and JNK1 siRNA (siJNK1) for 48 hours. The cell lysates were extracted from these cells to determine cellular levels of JNK1 and βactin (up panel) and quantitation analysis (bottom panel) as indicated. A representative blot from three independent determinations is shown.