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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: Gene. 2017 May 25;626:309–318. doi: 10.1016/j.gene.2017.05.048

Fig. 6. Repression of Brf1 decreases the induction of Pol III genes.

Fig. 6

(A) JNK1 and Brf1 siRNA reduce the levels of Brf1 in breast cells. MCF-7 cells were transfected with mismatch RNA (siMM), JNK1 siRNA (siJNK1) or Brf1 siRNA (siBrf1) 48 hours and then treated with ethanol for another 1 hour. The cell lysates were extracted from these cells to determine. Immuno-blots were performed for these sample to determine the cellular levels of Brf1. A representative blot from three independent determinations is shown (left panel) and quantitative analysis (right panel). (B–C) Pre-tRNALeu and 5S rRNA. HepG2-ADH cells and MCF-7 cells were transfected with siMM or siBrf1 for 48 hours and then treated with ethanol as described above. RNAs was derived from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (B and C, left panel), 5S rRNA (B–C, right panel), and GAPDH transcripts. The fold change was calculated by normalizing to the amount of GAPDH. *: P > 0.05. The values represent mean ± SE from three independent experiments.