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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: Gene. 2017 May 25;626:309–318. doi: 10.1016/j.gene.2017.05.048

Fig. 7. Down-regulating JNK1 and Brf1 expression decreases ethanol-induced anchorage-independent growth.

Fig. 7

(A) Alcohol increases the rate of liver cell transformation. Parent AML-12 cells and AML-12 cells expressing JNK1 siRNA or Brf1 siRNAs were poured in triplicate into 6-well plate with 0.35% agar containing 50mM ethanol, 20ng EGF or ethanol plus EGF; (B) Alcohol promotes transformation of breast cells. Parent MCF-10A cells and MCF-10A cells expressing JNK1 siRNA or Brf1 siRNAs were poured in triplicate into 6-well plate with 0.35% agar containing 25mM ethanol, 5nM E2 or ethanol plus E2. AML-12 cells or MCF-10A cells were incubated at 37°C in 5% CO2 for 3 weeks or longer and were fed with fresh complete media with or without ethanol, ethanol + EGF or ethanol + E2 twice weekly. Colonies were counted at 3 weeks after plating. The fold changes were calculated by normalizing to the colonies amounts control. Bar = 200 μM. *: P > 0.05. Values are the means ± SE (n ≥ 3).