Fig 7. Influence of ParB on the activities of PA0011 and PA0013 promoters.

(A) DNA sequences preceding PA0011 (PA0011p) and PA0013 (PA0013p) and their mutated versions, PA0011p_parS3mut and PA0013p_parS4mut, cloned upstream of promoter-less lacZ cassette in pPJB132 are presented. Putative promoters’ motifs, RBS sequences and start codons are indicated. Promoter -35 and -10 boxes were predicted using BPROM [74]. β-galactosidase activity was measured in extracts from PAO1161::araBADp (control strain) (B) and PAO1161::araBADp-flag-parB (ParB-overproducing strain) (C) cells carrying pPJB132 derivatives as indicated. Data represent mean activity from at least three cultures ±SD. *—p-value < 0.05 in two-sided Student’s t-test assuming equal variance.