Figure 5.

Insulin fails to normalize markers of β-cell dedifferentiation. A: Time course of changes in blood glucose during insulin (Ins) treatment in db/db mice (P = 0.0096 control [cont] vs. insulin). B: Islet cell number after 30-day treatment (P = 0.0875 control vs. insulin). C: Representative image of Foxo1/insulin coimmunostaining. D: Number of Foxo1-positive/insulin-positive cells. E: Representative image of Aldh1a3/insulin coimmunostaining. F: Number of Aldh1a3-positive/insulin-positive cells. Representative image (G) and proportion (H) of glucagon/insulin-positive cells. Data show means ± SEM. (n = 2–3 for each group.) P values were calculated by ANOVA for A, Mann-Whitney U test for B, and t test for E–H. *P < 0.05. AU, arbitrary units; Gcg, glucagon.