Figure 2.

Angiotensin-(1–7) is cleaved into several smaller peptides by neprilysin. Angiotensin-(1–7) and neprilysin were coincubated, and samples taken at various time points up to 48 h were analyzed by mass spectrometry. A: Extraction ion current areas for each peptide are expressed as a percentage of the total ion count at 48 h (n = 2). B: Based on mass spectrometry data, three neprilysin cleavage sites within angiotensin-(1–7) were identified. Data are the mean ± SEM. *P < 0.05 vs. all other cleavage products.