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. 2017 May 30;66(8):2201–2212. doi: 10.2337/db16-1318

Figure 5.

Figure 5

Angiotensin-(1–2) does not signal through the MasR to promote insulin secretion. pAKT and total AKT protein levels (A: n = 3–5) from C57BL/6.NEP−/− islets cultured in the absence or presence of 1 nmol/L angiotensin-(1–2), with or without pretreatment with 1 μmol/L A779. Western blot images are representative, with quantification of all experiments shown in the graphs. MasR mRNA levels in C57BL/6.NEP+/+ (B; n = 9) and C57BL/6.NEP−/− (D; n = 8) islets transfected with either si-Scramble or si-MasR for 72 h. Insulin secretion in response to 2.8 or 20 mmol/L glucose from transfected C57BL/6.NEP+/+ (C; n = 7) and C57BL/6.NEP−/− (E; n = 5) islets after a 48-h culture in the absence or presence of 1 nmol/L angiotensin-(1–2). Data are the mean ± SEM. #P < 0.001 vs. si-Scramble; *P < 0.05 vs. vehicle 20 mmol/L glucose.