Figure 3.

β-Cell lysate scaffolds recruit multiple hematopoietic lineages. A: Cumulative release of protein from PLG scaffolds loaded with β-cell lysate was analyzed over a period of 40 days. B: Hematoxylin-eosin staining of sections of β-cell and control scaffolds at day 14 postimplantation in NOD mice. Scale bar = 1 mm. C: FACS plots of hematopoietic lineages within β-cell and control scaffolds and spleens explanted 14 days postimplantation in 14-week-old NOD mice. Frequencies of dendritic cells (CD11c⁺), granulocytes (Mac1⁺ Gr.1⁺), monocytes (Mac1⁺ Gr.1⁻), B cells (CD19⁺), and CD4⁺, CD8⁺, and γδ-TCR⁺ T cells were determined. Plots were pregated on live CD45⁺ cells, and arrows within plots indicate subgating. Scaf^β-cell, β-cell scaffolds; Scaf^Ctrl, control scaffolds.