Figure 5.

T cells extracted from β-cell lysate scaffolds induce diabetes after adoptive transfer into NOD.SCID mice. β-Cell or control scaffolds were implanted in NOD mice for 14 days, and then scaffold-derived cells were isolated. A–C: T cells from β-cell scaffolds, control scaffolds, and spleens were adoptively transferred into NOD.SCID mice. Blood glucose levels in recipient mice were monitored weekly. D: Kaplan-Meier plot shows diabetes incidence in mice that received β-cell scaffold–derived cells, control scaffold–derived cells, or splenocytes. Mice with two consecutive readings of >250 mg/dL were considered diabetic. Scaf^β-cell, β-cell scaffolds; Scaf^Ctrl, control scaffolds.