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. 2017 Jul 21;96(29):e7111. doi: 10.1097/MD.0000000000007111

Figure 2.

Figure 2

PTEN regulates chemotherapy resistance to DDP in human NK/T-cell lymphoma SNK-6 cells. (A) The mRNA expression of PTEN in NK cells and SNK-6 cells were measured by real-time PCR. (B) Stable overexpression of PTEN in SNK-6 cells was carried out through lentivirus transfection while knockdown of its expression was performed using shRNA. The mRNA expression of PTEN was measured by real-time PCR. (C) The protein expression of PTEN in NK cells and SNK-6 cells were measured by Western blot. (D) The protein expression of PTEN in overexpression and knowdown SNK-6 along with control cells were measured by Western blot. (E) Cells were cultured for 5 days and cell proliferation was examined by CCK-8. (F) Cells were treated with DDP (0.03125–8 μg/mL) for 24 hours. Cell viability was examined by CCK-8. Lenti-con (cells transfected with control vector for PTEN overexpression); Lenti-Shcon (cells transfected with control shRNA vector); Lenti-PTEN (cells transfected with vector for PTEN overexpression); Lenti-Shcon (cells transfected with shRNA vector). CCK-8 = cell counting Kit-8, DDP = different doses of cisplatin, NK/T-cell = nasal-type natural killer/T-cell, PTEN = phosphatase and tensin homolog.