Fig. 2.
Maximum – likelihood phylogeny based on 16S rRNA gene sequences (846 bp) showing the relationships among representative Bradyrhizobium isolates from Mozambique (in bold) with Bradyrhizobium type (T) and reference strains used in commercial inoculants, B. elkanii SEMIA 587 and SEMIA 5019, B. japonicum SEMIA 5079, and B. diazoefficiens SEMIA 5080 and USDA 110 (with an asterix). Ensifer fredii USDA 205T was inluded as an outgroup. The evolutionary history was inferred using the nearest-neighbor-interchange method. Only bootstrap confidence levels >55% are indicated at the internodes. The scale bar indicates 1 substitution per 100 nucleotides. A and B represent clades B. japonicum and B. elkanii, respectively; a and b represent the clustering of the five best nitrogen fixer strains from Mozambique.