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. 2017 Apr 6;8(26):42098–42115. doi: 10.18632/oncotarget.16887

Figure 3.

Figure 3

(A) The effect of different concentrations of HMGB1 on MSC expansion. (B) The DNA content of MSCs in the gelatin sponge and HMGB1-gelatin sponge scaffolds after 0, 2, 4, 6 and 8 days of culture. Each scaffold was seeded with 100 μL of cells to achieve a density of 1 × 106 cells/scaffold. *Significant (P < 0.05) difference between the HMGB1-gelatin and gelatin sponge scaffolds at the same time point. (C) Confocal images of the distribution of CM-DiI-stained MSCs in (C) the gelatin sponge and (D) the HMGB1-gelatin sponge scaffolds obtained after 7 days of in vitro culture. Three-dimensionally rendered z-stack images were evaluated.